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In vitro testing of the impact of age on human BMSC-mediated <t>CD34</t> + HSPCs migration. (A) Schematic representation of the experimental setup, including (i) a timeline of the principal experimental steps, (ii) a graphic description of the different experimental conditions and (iii) a representative image showing migrated CD34 + HSPCs (red) in the different experimental conditions. (B) Age-dependent effects of mobilizing agents on CD146 + BMSC-mediated CD34 + HSPC transmigration; each graph represents an individual donor with its technical replicates, express as percentage of migrated cells relative to the internal reference (without CD146 + BMSCs). N = 3 subjects per group, with 3 technical replicates per subject. Statistical analysis was performed using the Kruskal–Wallis test. * p < 0.05; ** p < 0.01. Negative control, NC; hematopoietic stem progenitor cells, HSPCs; bone marrow stromal cells, BMSCs; untreated control, CTRL; specific CXCR4 inhibitor, AMD3100; granulocyte colony-stimulating factor, G-CSF; norepinephrine, NE. (Panel A (ii) and (iii) created in https://BioRender.com ).
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In vitro testing of the impact of age on human BMSC-mediated CD34 + HSPCs migration. (A) Schematic representation of the experimental setup, including (i) a timeline of the principal experimental steps, (ii) a graphic description of the different experimental conditions and (iii) a representative image showing migrated CD34 + HSPCs (red) in the different experimental conditions. (B) Age-dependent effects of mobilizing agents on CD146 + BMSC-mediated CD34 + HSPC transmigration; each graph represents an individual donor with its technical replicates, express as percentage of migrated cells relative to the internal reference (without CD146 + BMSCs). N = 3 subjects per group, with 3 technical replicates per subject. Statistical analysis was performed using the Kruskal–Wallis test. * p < 0.05; ** p < 0.01. Negative control, NC; hematopoietic stem progenitor cells, HSPCs; bone marrow stromal cells, BMSCs; untreated control, CTRL; specific CXCR4 inhibitor, AMD3100; granulocyte colony-stimulating factor, G-CSF; norepinephrine, NE. (Panel A (ii) and (iii) created in https://BioRender.com ).

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Impact of aging on CD146 + mesenchymal stromal cells-mediated regulation of bone marrow CD34 + hematopoietic stem/progenitor cell mobilization

doi: 10.3389/fbioe.2026.1802093

Figure Lengend Snippet: In vitro testing of the impact of age on human BMSC-mediated CD34 + HSPCs migration. (A) Schematic representation of the experimental setup, including (i) a timeline of the principal experimental steps, (ii) a graphic description of the different experimental conditions and (iii) a representative image showing migrated CD34 + HSPCs (red) in the different experimental conditions. (B) Age-dependent effects of mobilizing agents on CD146 + BMSC-mediated CD34 + HSPC transmigration; each graph represents an individual donor with its technical replicates, express as percentage of migrated cells relative to the internal reference (without CD146 + BMSCs). N = 3 subjects per group, with 3 technical replicates per subject. Statistical analysis was performed using the Kruskal–Wallis test. * p < 0.05; ** p < 0.01. Negative control, NC; hematopoietic stem progenitor cells, HSPCs; bone marrow stromal cells, BMSCs; untreated control, CTRL; specific CXCR4 inhibitor, AMD3100; granulocyte colony-stimulating factor, G-CSF; norepinephrine, NE. (Panel A (ii) and (iii) created in https://BioRender.com ).

Article Snippet: The following antibodies were used: PDGFRB/CD140b-APC-Vio770 (Miltenyi, cat # 130-129–294), CD146-PE-Vio615 (Miltenyi, cat # 130-125–731), CD45-VioBright B515 (Miltenyi, cat # 130-110–640), CD90-PE-Vio770 (Miltenyi, cat # 130-114–862), CD31-VioBlue (Miltenyi, cat # 130-110–674), and CD34-PE (Miltenyi, cat # 130-120–515).

Techniques: In Vitro, Migration, Transmigration Assay, Negative Control, Control